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COVID-FLU-RSV RT PCR Detection kit
Molecular test for the qualitative detection of SARS-CoV-2, Influenza A/B ed RSV A/B RNA in upper and lower respiratory samples during the acute phase of the infection
The positive result is indicative of the presence of SARS-CoV-2 RNA and/or Influenza A/B RNA and/or RSV A/B RNA. Clinical correlation with the patient’s history and other diagnostic information are needed to determine the patient’s infectious status.
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Principle of the assay
The COVID-FLU-RSV RT PCR Detection kit is a one-step multiplex real-time RT-PCR assay with fluorescent probes. The method allows the detection of the specific viral genes ORF1b/RdRp, S and N for SARS-CoV-2 and of the specific genes for Influenza A and B and RSV A and B starting from the same sample.
The kit consists of all the reagents required for the analysis. Positive and negative PCR controls are included.
Selling Points
![ico-utilizzo](https://www.diathevacovid-19.com/en/wp-content/uploads/sites/2/2020/04/ico-utilizzo.png)
Ease of use
![ico-compatibilita PCR detection kit Covid-19 - DIATHEVA - Compatibilità: risultati altamente riproducibili su diversi strumenti](https://www.diathevacovid-19.com/en/wp-content/uploads/sites/2/2020/04/ico-compatibilita.png)
Open system: the kits are compatible and can be used with different extraction systems, including automated ones and on the most popular real-time PCR platforms
![ico-stoccaggio](https://www.diathevacovid-19.com/en/wp-content/uploads/sites/2/2020/04/ico-stoccaggio.png)
Reaction setup at room temperature
![ico-affidabilita Covid-19 PCR detection kit - DIATHEVA - L’amplificazione del controllo interno RNAsi P consente di valutare l’estrazione dell’RNA e identificare la presenza di inibitori della PCR](https://www.diathevacovid-19.com/en/wp-content/uploads/sites/2/2020/04/ico-affidabilita.png)
Reliability: the amplification of the internal control RNAse P allows to evaluate sample quality, RNA extraction and to identify the presence of PCR inhibitors
![ico-specificita](https://www.diathevacovid-19.com/en/wp-content/uploads/sites/2/2020/04/ico-specificita.png)
Diagnostic sensitivity: 100% – Diagnostic specificity: 100%
Workflow
![raccolta](https://www.diathevacovid-19.com/en/wp-content/uploads/sites/2/2020/04/raccolta.jpg)
1. Sample collection
Upper and lower respiratory samples
![estrazione](https://www.diathevacovid-19.com/en/wp-content/uploads/sites/2/2020/04/estrazione.jpg)
2. RNA extraction
RNA isolation and purification
![preparazione](https://www.diathevacovid-19.com/en/wp-content/uploads/sites/2/2020/04/preparazione.jpg)
3. RT-PCR Setup
Add the RNA sample and controls to the PCR tubes containing the reagents for amplification
![real-time](https://www.diathevacovid-19.com/en/wp-content/uploads/sites/2/2020/04/real-time.jpg)
4. Real Time RT-PCR
Amplification by RT-PCR
![analisi](https://www.diathevacovid-19.com/en/wp-content/uploads/sites/2/2020/04/analisi.jpg)
5. Results analysis
Workflow
![raccolta](https://www.diathevacovid-19.com/en/wp-content/uploads/sites/2/2020/04/raccolta.jpg)
1. Sample collection
Upper and lower respiratory samples
![estrazione](https://www.diathevacovid-19.com/en/wp-content/uploads/sites/2/2020/04/estrazione.jpg)
2. RNA extraction
RNA isolation and purification
![preparazione](https://www.diathevacovid-19.com/en/wp-content/uploads/sites/2/2020/04/preparazione.jpg)
3. RT-PCR Setup
Add the RNA sample and controls to the PCR tubes containing the reagents for amplification
![real-time](https://www.diathevacovid-19.com/en/wp-content/uploads/sites/2/2020/04/real-time.jpg)
4. Real Time RT-PCR
Amplification by RT-PCR
![analisi](https://www.diathevacovid-19.com/en/wp-content/uploads/sites/2/2020/04/analisi.jpg)