COVID-FLU-RSV RT PCR Detection kit

Molecular test for the qualitative detection of SARS-CoV-2, Influenza A/B ed RSV A/B RNA in upper and lower respiratory samples during the acute phase of the infection

The positive result is indicative of the presence of SARS-CoV-2 RNA and/or Influenza A/B RNA and/or RSV A/B RNA. Clinical correlation with the patient’s history and other diagnostic information are needed to determine the patient’s infectious status.

Le certificazioni del nostro Covid-19 PCR detection kit - DIATHEVA

Principle of the assay

The COVID-FLU-RSV RT PCR Detection kit is a one-step multiplex real-time RT-PCR assay with fluorescent probes. The method allows the detection of the specific viral genes ORF1b/RdRp, S and N for SARS-CoV-2 and of the specific genes for Influenza A and B and RSV A and B starting from the same sample.
The kit consists of all the reagents required for the analysis. Positive and negative PCR controls are included.

Selling Points

Ease of use

PCR detection kit Covid-19 - DIATHEVA - Compatibilità: risultati altamente riproducibili su diversi strumenti

Open system: the kits are compatible and can be used with different extraction systems, including automated ones and on the most popular real-time PCR platforms

Reaction setup at room temperature

Covid-19 PCR detection kit - DIATHEVA - L’amplificazione del controllo interno RNAsi P consente di valutare l’estrazione dell’RNA e identificare la presenza di inibitori della PCR

Reliability: the amplification of the internal control RNAse P allows to evaluate sample quality, RNA extraction and to identify the presence of PCR inhibitors

Diagnostic sensitivity: 100% – Diagnostic specificity: 100%

Workflow

1. Sample collection
Upper and lower respiratory samples

2. RNA extraction
RNA isolation and purification

3. RT-PCR Setup
Add the RNA sample and controls to the PCR tubes containing the reagents for amplification

4. Real Time RT-PCR
Amplification by RT-PCR

5. Results analysis

Workflow

1. Sample collection
Upper and lower respiratory samples

2. RNA extraction
RNA isolation and purification

3. RT-PCR Setup
Add the RNA sample and controls to the PCR tubes containing the reagents for amplification

4. Real Time RT-PCR
Amplification by RT-PCR

5. Results analysis

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